Xmnl RFLP at 5q13 detected by a 0.49 kb Xmn I fragment of human hexosaminidase (HEXB) cDNA

RFLP detected at the 8924 locus by a thyroglobulin cDNA probe

SOURCE : 1660 bp corresponding to two contiguous Pst I fragments from human thyroglobulin gene cDNA cloned in pBR 322 (ref. I). This cDNA probe is located Ca 100 Kb 3 1 from PCHT 16/3.2-8.0 probe that reveals polymorphic fragments previously described by Baas et al. (ref.II). POLYMORPHISM : PvuII identifies a single two allele polymorphism Al : 13.2 Kb A2 : 4.4 Kb Mst II identifies a single two...

Identification of Avian Salmonella Isolates by PCR-RFLP Analysis of a fliC Gene Fragment

The genus of Salmonella is very polymorphic and comprised of a number of genetically closely related serotypes. It is one of the emerging pathogen in food-borne disease which is often found in contaminated chicken eggs. Salmonella enterica is considered one of the major pathogens in public health worldwide. A total of 31 Salmonella isolates identified by specific antisera, which included Salmon...

Sac I Restriction Fragment Length Polymorphism (RFLP) related to the human CST2 gene.

Restriction Fragment Length Polymorphism (RFLP) related to cystatin gene (CST) family was detected in the Japanese population by using restriction enzyme Sac I. A polymorphic site, located at 0.9 kb from the 3' end of the CST2 gene, revealed a two allele polymorphism with band sizes of 3.5 kb and 8.3 kb by hybridization with probe including exon 2 of the CST1 gene. The gene frequencies in the J...

Human cDNA probe (OL167,D21S109) detects RFLP on chromosome 21.

Restriction Fragment Length Polymorphism (RFLP)

Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...